Flow Cytometry Facility
The Flow Cytometry Facility provides investigators with equipment and support for cell sorting (separation), acquisition, and analysis of flow cytometric data with a variety of state-of-art multicolor flow cytometry instruments.
This Facility is managed by the Institute for Research in Biomedicine (IRB): https://irb.usi.ch/facilities/flow-cytometry/
Equipment
Cell sorter
- Cell sorting is performed on a SORP FACSymphony S6 equipped with five lasers (488, 561, 640, 355, and 405 nm excitation wavelengths) and 28 fluorescence detection channels. It can perform high-speed sorting (up to 20, 000 events/sec) with high purity (up to 99%)
Benchtop analyzers
- FACSymphony A5, equipped with 5 lasers (488, 561, 640, 355, and 405 nm excitation wavelengths) and 28 fluorescent channels
- LSRFortessa, equipped with four lasers (488, 561, 640, and 405 nm excitation wavelengths) and 16 fluorescence channels
- FACSCantoII (488, 640 and 405 nm lasers-eight colors, equipped with a high throughput system to acquire samples from 96 or 384 well plates)
- FACSCanto I (488 and 640 nm lasers-six colors), equipped with a high throughput system to acquire samples from 96 or 384 well plates
Support
- Individual training on bench-top analyzers
- Cell sorting
- Maintenance of all instruments
- Assistance with experimental design
- Assistance with data analysis
- Assistance with troubleshooting
