Protein Production Facility
The IRB has developed relevant competence in the field of protein-protein and antigen-antibody interaction applied to the study of inflammatory and infectious diseases. Furthermore, it continuously develops and evaluates new techniques for protein production and purification.
The facility is equipped with state-of-art technologies for small to medium-scale purification from cell-free systems, bacteria, insect, and mammalian cell lines. It currently specializes in using the baculovirus expression system and mammalian expression, especially for the production of multimeric protein complexes. Depending upon the level of expression of a given protein, quantities ranging from milligrams to grams can be produced using resources at the facility. Finally, the facility is centralizing stocks of a large number of expression vectors for bacterial, insect and mammalian cells.
This Facility is managed by the Institute for Research in Biomedicine (IRB): https://irb.usi.ch/facilities/protein-production/
Equipment
- Protein purification system
- ÄKTA™purifier
- Measuring binding affinity
- Monolith NT.115 Pico
- Circular dichroism spectrometer
- Chirascan™ V 100 circular dichroism
- Semi-automated benchtop TFF system
- Cogent uScale tangential flow filtration system
Support
- Help with cellular biochemistry, protein expression and purification
- Assistance and information to all laboratories and researchers
