Experimental Pathology Facility
Head of Experimental Pathology Facility
Simone Mosole
simone.mosole@ior.usi.ch
Members
Cristina Dongilli, Lab Technician
Open positions
Overview
The Experimental Pathology Facility offers extensive technical and scientific expertise to support the pathology requirements of scientists, ensuring consistent and reliable results.
The Experimental Pathology is of interest for:
- supplying routinary histologic and immunophenotypic techniques, evaluating the more suitable procedure based on the expected results (tissues, cells and organoids)
- identification and evaluation of experimentally induced lesions in animal models and human specimens
- phenotyping of transgenic mouse models
- evaluation of experimental model correlating the animal models with the human physiologic and/or pathologic counterpart
- RNA in situ hybridization (ISH) assay (RNAscope) based on signal amplification and background suppression technology which advances RNA biomarker analysis and localization in tissues, cells and organoids
- Multiplex analyses for the characterization of the tumor microenvironment in murine and human specimens
Equipment
The Experimental pathology service is fully equipped to perform semi-automated processing of FFPE tissue sections, with a panel of staining procedures ranging from conventional histochemical methods to molecular characterization using standard or customized antibodies in immunohistochemistry. Processed samples can be digitally acquired and stored for image processing and morphometric analysis with an Aperio AT2 pathology digital scanner.
List of equipment:
Leica Histocore Pearl
The HistoCore PEARL tissue processor provides reliability with a new retort design, and allows safe and reliable conventional processing with a 200 cassette per run capacity.
Leica Histocore Arcadia H+C
The HistoCore Arcadia modular tissue embedding system offers the flexibility to arrange the embedding workflow allows for simple operation and precise control; resulting in a smooth workflow, reliability and speed of the embedding work.
Leica RM2265
Leica RM2265 microtome deliver superior sectioning across a spectrum of specimen types, from soft to hard, and spanning routine biomedical research to specialized applications.
Leica Aperio AT2
The Aperio AT2 is designed with an autoloader capacity for up to 400 slides, minimizing glass movement and manipulation, keeping the slides safe and delivering an unparalleled time-to-view speed and high quality digital slides.
MACSima
The MACSima™ is a fully automated imaging system based on fluorescence microscopy. It operates through iterative cycles of stainings, sample washing, multi-channel image acquisition and signal bleaching. These cycles allow to obtain a quantity (theoretically up to 400 markers versus 4/5 markers for a manual IF) and quality (standardization and less variability) of data that is significantly superior to classic IF protocols, without affecting the structural integrity of the samples in analysis (FFPE, adherent cells, suspension cells, organoids).
Facility Acknowledgement
Please acknowledge the Experimental Pathology Facility in any scientific publication or oral presentation for which data was generated with the use of our equipment, our services, or with the help of our staff’s expertise.
To demonstrate that the equipment in the Facility is used to generate publishable results, please send us a copy of your publication that includes data obtained from the use of our facility.
Co-authorship
If a member of the Facility has made a significant intellectual contribution to your publication beyond routine sample analysis, you should consider co-authorship.
